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  • br Conclusions br In conclusion


    5. Conclusions
    In conclusion, our study indicated that circVAPA was significantly up-regulated in CRC patients’ tissues and Navitoclax and associated with unfavorable clinicopathologic features. CircVAPA could serve as a
    promising non-invasive biomarker for CRC detection. CircVAPA pro-moted CRC progression by sponging miR-101 and could serve as a potential therapeutic target for CRC treatment.
    This work was supported by the National High-Tech R & D Program of China (863 Program) (2015AA033602); 1351 Personnel Training Program of Beijing Chao-Yang Hospital Affiliated to Capital Medical University (CYXZ-2017-09).
    Competing interests
    The authors declare that they have no competing interests.
    Appendix A. Supplementary data
    Contents lists available at ScienceDirect
    Biomedicine & Pharmacotherapy
    journal homepage:
    Circular RNA circ_0002138 is down-regulated and suppresses cell proliferation in colorectal cancer 
    Haoyu Ruana,1, Xuan Denga,1, Liu Donga, Daming Yangb, Ying Xub, Haixia Pengb, , Ming Guana,c,d,
    a Department of Clinical Laboratory, Huashan Hospital, Fudan University, 200040, China
    b Digestive Endoscopy Center, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 XianXia Road, Shanghai 200336, China
    c Central Laboratory, Huashan Hospital, Fudan University, Shanghai 200040, China
    d Department of Clinical Laboratory, Huashan Hospital North, Fudan University, Shanghai, 201907, China
    Circular RNAs (circRNAs) have been recently identified as widespread and diverse endogenous noncoding RNAs that may harbor vital functions in humans. However, the role of circRNAs in the process of tumorigenesis and development of colorectal cancer (CRC) remains hitherto vague. In this study, we investigated the expression level of circ_0002138 in 35 paired CRC tissues by quantitative real-time polymerase chain reaction (qRT-PCR) and found that circ_0002138 was stably down-regulated in CRC tissues compared to paired adjacent normal tissues (P < 0.001). Fisher's exact test was further conducted to analyze the relationship between circ_0002138 expression level and clinico pathological factors of CRC patients. Circ_0002138 expression was significantly correlated with age. To evaluate the diagnostic value of circ_0002138, a receiver operating characteristic (ROC) curve was used and the area under the ROC curve was 0.7249. Additionally, functional analysis demonstrated that circ_0002138 significantly inhibited CRC cell proliferation in vitro. Overall, our data suggest that circ_0002138 may become a novel potential biomarker for diagnosis and treatment target of CRC.
    1. Introduction
    Colorectal cancer (CRC) is the third most commonly diagnosed cancer in the world and the fourth principal cause of cancer deaths worldwide [1]. The 5-year survival rate of patients with CRC was 90.1% for patients at early stage and only 11.7% for patients with distant metastasis [2]. To improve the diagnosis and treatment response of patients, a better understanding of molecular mechanisms underlying the development of CRC is clinically important.
    Circular RNAs (circRNAs) are a class of non-coding RNAs char-acterized by covalently closed continuous loops with neither 5′ to 3′ polarity nor a polyadenylated tail [3]. Compared with linear RNA, circRNAs are usually stable, abundant, conserved and tissue/develop-mental-stage specific [4,5]. These remarkable characteristic makes them to be the potantial ideal molecular markers of CRC. It has been reported that circRNAs serve as microRNA (miRNA) sponges and RNA-binding protein (RBP) sequestering agents as well as transcription regulators to regulate gene expressions [6–9]. However, little is known