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  • Pam3CSK4 br Zlobec I Suter G Perren A Lugli


    [27] Zlobec I, Suter G, Perren A, Lugli A. A next-generation tissue microarray (ngTMA) protocol for biomarker studies. J Vis Exp 2014(91).
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    Research paper Co-expression profiling of plasma miRNAs and long noncoding RNAs in T
    gastric cancer patients
    Hamid Ghaedia, Mohammad Amin Nazer Mozaffarib, Zaker Salehic, Hassan Ghasemid, Seyed Sajjad Zadiane, Sadegh Alipoorf, Shiva Hadianpourg, Behnam Alipoorg,
    a Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
    b Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
    c Department of Radiation Sciences, Yasuj University of Medical Sciences, Yasuj, Iran
    d Department of Clinical Biochemistry, Abadan School of Medical Sciences, Abadan, Iran
    e Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
    f Department of Nutrition, School of Health, Yasuj University of Medical Sciences, Yasuj, Iran
    g Department of Laboratory Sciences, Faculty of Paramedicine, Yasuj University of Medical Sciences, Yasuj, Iran
    Gastric cancer
    Purpose: The recent researches indicate that differential non-coding RNAs Pam3CSK4 signatures could be as-sociated with the pathogenesis of gastric cancer (GC). However, there are few studies focused on lncRNA-miRNAs co-expression profiling in GC patients. Therefore, in the present study the expression of H19 and MEG3 and their related miRNAs including miR-148a-3p, miR-181a-5p, miR-675-5p and miR-141-3p were determined in the plasma samples of GC patients and controls.
    Materials and methods: This case-control study included 62 GC patients and 40 age- sex matched controls. The non-coding RNA levels were assessed by real-time PCR. Further, using in silico analysis, we identified shared targets of studied miRNAs and performed GC-associated pathway enrichment analysis.
    Conclusion: The results of current study demonstrated that combination of H19, MEG3 and miR-675-5p ex-pression levels could provide a potential diagnostic panel for GC.
    1. Introduction
    Gastric Cancer (GC) is the fourth most common cancer and the second cause of cancer mortality worldwide (Rahman et al., 2014; Malekzadeh et al., 2009) with an estimated 1 million new cases and 738,000 deaths globally in 2008 (Arita et al., 2013). Despite its redu-cing incidence in many countries, GC still remains a significant public health problem in Iran (Rahman et al., 2014). It has been shown that GC is the most common cancer in north and northwest Iran
    (Malekzadeh et al., 2009). GC is a heterogeneous and multifactorial malignancy and its incidence is associated with the several environ-mental factors including Helicobacter pylori infection, diet, smoking and also host genetic susceptibility (Tan and Yeoh, 2015). Despite a growing understanding of molecular mechanisms occurring during GC development, there are no reliable biomarkers to allow early detection of which. Nonetheless, accumulated evidence suggests that aberrant non-coding RNAs (ncRNAs) expression may be associated whit GC pa-thogenesis and could be used as the potential therapeutic targets and
    Abbreviations: GC, Gastric Cancer; ncRNAs, Non-coding RNAs; lncRNAs, Long-noncoding RNAs; miRNA, microRNA; MEG3, maternally expressed 3; ROC, Receiver Operating Characteristic; AUC, Area Under the ROC curve
    Corresponding author.
    E-mail address: [email protected] (B. Alipoor).
    ncRNAs represent the majority of the human genome. Based on the length, they are divided into two major categories including small ncRNAs -such as miRNAs (miRNAs)- and long ncRNAs (lncRNAs) (Hombach and Kretz, 2016). Recently, several investigations suggest that H19 lncRNAs could inhibit apoptosis and promoted the prolifera-tion of GC cells (Yan et al., 2017). This lncRNA is capable of generating miR-675, which is responsible for the regulation of H19 function and differentially expressed in many human tumors (Yan et al., 2017; Cai and Cullen, 2007; Liu et al., 2016). In addition, H19 lncRNAs could act as endogenous sponge for miR-141 and indirectly affect its targets in GC related signaling pathway (Zhou et al., 2015a).
    Another well-known lncRNA, maternally expressed gene 3 (MEG3), is reported to be deregulated in GC tissues and the aberrant expression of that could be related to the GC pathogenesis (Sun et al., 2014; Wei and Wang, 2017). Interestingly, recent finding has revealed an inter-action between MEG3 and some miRNAs in GC. This lncRNA functioned as a “molecular sponge” for miR-181, miR-148a and miR-141 and thereby modulates the inhibitory effects of these miRNAs on the ex-pression of target genes (Peng et al., 2015; Yan et al., 2014; Zhou et al., 2015b).