Archives

  • 2022-09
  • 2022-08
  • 2022-07
  • 2022-06
  • 2022-05
  • 2022-04
  • 2021-03
  • 2020-08
  • 2020-07
  • 2020-03
  • 2019-11
  • 2019-10
  • 2019-09
  • 2019-08
  • 2019-07
  • br Cytotoxic e ect o http www glpbio com simage

    2022-08-17


    3.6. Cytotoxic effect of C. Curetum fractions
    4. Discussion
    Herbal products have been utilized for therapeutic purposes for time
    Fig. 4. α-amylase inhibitory activity of the C. curetum hexane, acetone, me-thanol and aqueous fractions in addition to Acarbose.
    immemorial, and to this day, many important and familiar medica-ments originate from plants [34]. Throughout the phytochemical screening outcomes, it was observed that the polar fractions (methanol and aqueous) were rich in polyphenols. On the other hand, the hexane and acetone fractions contained steroidal metabolites. Moreover, the quantitative phytochemical tests results revealed the absence of phe-nolic, flavonoid and tannin contents in the hexane and acetone frac-tions, while the aqueous fraction contained the highest contents of flavonoids, tannins, and phenols.
    Diabetes mellitus is a chronic disorder that becomes a dangerous global health problem and considered one of the main risks of neuro-pathy, stroke, nephropathy, retinopathy, and cataracts. Some natural and synthetic products may be used as potent inhibitors of carbohy-drate degrading PMSF such as α-amylase and α-glucosidase to de-crease the risk of diabetes, especially type 2 diabetes and its serious complications.
    In fact, α-amylase and α-glucosidase are key enzymes that have been major drug targets for the development of therapies to treat dia-betes and to reduce the severity of complications. Thus, the current investigation assessed the α-amylase and α-glucosidase activities of the four fractions derived from C. curetum aerial parts using standard bio-chemical laboratory methods.
    The results showed that the aqueous and methanol fractions had
    α-amylase inhibitory activity and IC50 values of the C. curetum hexane, acetone, methanol and aqueous fractions in addition to the reference drug Acarbose.
    Conc. Acarbose Hexane fraction Acetone fraction Methanol fraction Aqueous fraction
    Table 4 The α-glucosidase inhibitory activity and IC50 values of the C. curetum hexane, acetone, methanol and aqueous fractions in addition to Acarbose.
    Conc. Acarbose Hexane fraction Acetone fraction Methanol fraction Aqueous fraction
    Fig. 5. α-glucosidase inhibitory activity of the C. curetum hexane, acetone, methanol and aqueous fractions in addition to Acarbose.
    strong α-amylase enzyme inhibitory activity in comparison with the reference anti-diabetic drug Acarbose with IC50 values of 21.37 ± 0.31 and 30.2 ± 0.42 μg/mL, respectively; the Acarbose anti-amylase IC50 value was 28.18 ± 0.42 μg/mL. These results indicate gonadotropins the aqueous fraction of C. curetum has powerful α-amylase enzyme inhibitory ac-tivity, even more so than the commercially available antidiabetic drug Acarbose, while the methanolic fraction had nearly the same anti-diabetic activity as Acarbose. However, assessing the α-glucosidase inhibitory activity of the four C. curetum fractions revealed that the methanolic fraction had potent antiglucosidase property, greater than that of Acarbose with an IC50 value of 27.54 ± 4.28 μg/mL; the Acarbose α-glucosidase IC50 value was 37.15 ± 0.33 μg/mL.
    The antidiabetic activity of C. curetum aqueous and methanolic fractions indicated that the phenolic, flavonoid and tannins contents may inhibit α-amylase and α-glucosidase. A study conducted by Kalita et al. showed that the phenolic content in potato inhibits the activity of α-amylase and α-glucosidase [35]. In another study performed by Salehi et al., there was a positive correlation between a plant’s poly-phenolic contents and α-amylase and α-glucosidase inhibitory activity [36].