br The presented reconstruction technique is of general use and
The presented reconstruction technique is of general use and can be applied in the reconstruction of different cell types, and even in other staining technologies. The analysis can be performed in a timely and cost-effective manner. Therefore, the proposed au-tomatic segmentation method offers considerable promise for the automating analysis. The computer program developed for apply-ing the presented reconstruction procedure can substitute human medical experts in this tedious and error-prone work, delivering results that are acceptable in medical practice within a substan-tially shorter time.
Future research in this field will be directed toward the follow-ing tasks:
• The proposed method will be implemented in hospital practice. Additional experiments on a higher number of images repre-senting the enlarged Peroxynitrite of patients will be required.
• The set of typical cell patterns corresponding to the true cell types will be expanded, based on which reconstruction will be performed.
• Future investigations will focus on applying the method to other staining technologies, such as Ki-67 or ER/PR staining, which are used in different types of pathology.
• Additional efforts will be directed at accelerating the computa-tion by implementing parallel processing of the data.
Declaration of interests
The authors declare that they have no known competing finan-cial interests or personal relationships that could have appeared to influence the work reported in this paper.
Credit authorship contribution statement
T. Les: Conceptualization, Formal analysis, Investigation, Methodology, Software, Validation, Writing - original draft. T. Markiewicz: Conceptualization, Methodology, Project administra-tion, Resources, Supervision. S. Osowski: Methodology, Writing - original draft, Writing - review & editing. M. Jesiotr: Data curation, Resources, Validation.
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Autophagy as a molecular target for cancer treatment